期刊论文

Wu, TB

英文

Biosensors and Bioelectronics

0956-5663

2024

247

115936

10.1016/j.bios.2023.115936

CRediT authorship contribution statement Zhujun Liu: Data curation, Formal analysis, Investigation, Methodology, Visualization, Writing – original draft, Writing – review & editing. Jie Xu: Data curation, Investigation, Writing – review & editing. Shan Huang: Data curation, Formal analysis. Wei Dai: Data curation. Wei Zhang: Project administration. Longjie Li: Methodology. Xianjin Xiao: Methodology, Project administration. Tongbo Wu: Conceptualization, Formal analysis, acquisition, Investigation, Methodology, Project

本校为其他机构

Gene point mutations play a significant role in the development of cancer. Therefore, developing a sensitive, specific, and universally applicable method for detecting gene point mutation is crucial for clinical diagnosis, prognosis, and cancer treatment. Recently, gene point mutation detection methods based on CRISPR/Cas12a detection have emerged. However, existing methods generally lack universality and specificity. In this study, we have developed a CRISPR/Cas12a-based method that combines improved allele-specific polymerase chain reaction and single base extension to translate the point mu...