In this paper,the biosynthetic process is monitored under different fermentation conditions,and the product is characterized by electron microscopy,X-ray energy dispersive spectrometer photoelectron(EDS),and X-ray photoelectron spectroscopy(XPS).The results show that:ⅰ)Nano-selenium(nano-Se)is synthesized in the cytoplasm of Pseudomonas sp.J1;ⅱ)Glutathione plays an important role in nano-Se biosynthesis;ⅲ)When the concentration degree of sodium selenite is higher,it becomes more toxic to Pseudomonas sp.J1;and ⅳ)The reduction process in Pseu...