Technical note: Isolation and characterization of porcine mammary epithelial cells,

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成果类型：

期刊论文

作者：

Dahanayaka, S.;Rezaei, R.;Porter, W. W.;Johnson, G. A.;Burghardt, R. C.;...

通讯作者：

Wu, G.

作者机构：

[Hou, Y. Q.; Wu, G.; Bazer, F. W.; Rezaei, R.; Dahanayaka, S.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

[Porter, W. W.; Burghardt, R. C.; Johnson, G. A.] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.

[Hou, Y. Q.; Wu, G.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.

[Wu, G.; Wu, Z. L.] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.

通讯机构：

[Wu, G.] T

Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.

语种：

英文

关键词：

beta-casein;characterization;isolation;lactation;mammary epithelial cells;swine

期刊：

Journal of Animal Science

ISSN：

1525-3163

年：

2015

卷：

期：

页码：

5186-5193

DOI：

10.2527/jas.2015-9250

基金类别：

Texas A&M AgriLife Research [H-8200]; Hubei Hundred Talent program at Wuhan Polytechnic University; China Scholarship CouncilChina Scholarship Council

机构署名：

本校为其他机构

院系归属：

动物科学与营养工程学院

摘要：

Within the mammary gland, functional synthesis of milk is performed by its epithelial (alveolar) cells. The availability of a stable mammary epithelial cell line is essential for biochemical studies to elucidate cellular and molecular mechanisms responsible for nutritional regulation of lactation. Therefore, porcine mammary epithelial cells (PMEC) were isolated from mammary glands of a 9-mo-old nonpregnant and nonlactating gilt and cultured to establish a nonimmortalized cell line. These cells were characterized by expression of cytokeratin-18 ...

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Technical note: Isolation and characterization of porcine mammary epithelial cells,

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