期刊论文

Xu, S.-Q.

中文

中国组织工程研究

2095-4344

2005

9

34

160-162

国家自然科学基金资助（30170051）~~；

本校为第一机构

背景:近年来抗乙肝免疫核糖核酸作为一种免疫治疗剂,对慢性肝炎的治疗作用备受重视,但临床应用效果不稳定,因此构建一个能有效保护免疫核糖核酸并能被组织更好吸收的免疫核糖核酸运载体系具有重要意义。壳聚糖作为一种新辅料取得了良好的效果。目的:制备抗乙肝免疫核糖核酸的壳聚糖纳米载体,观察其免疫活性。方法:实验于2003-12/04-12在华中科技大学同济医学院环境医学研究所完成。①制备壳聚糖-免疫核糖核酸纳米粒采用复凝聚方法。②壳聚糖-免疫核糖核酸纳米粒形态、粒径和表面电位测定采用扫描电镜、原子力显微镜、Zeta电位/粒度分析仪观察。③壳聚糖对包裹的免疫核糖核酸的保护作用观察采用核...

Background: In recent years, being a kind of immune therapeutic form, hepatitis B immune ribonucleic acid (iRNA) has drawn a great attention in treatment of chronic hepatitis B. But its clinical effects are not stable, for which, it is significant to construct iRNA carrier system that can not only protect RNA effectively, but also be well absorbed by tissues. Being a kind of new supplementary material, chitosan has provided good results. Objective: To observe the immune activity of anti-hepatitis B iRNA by preparing its chitosan nanocarrier. Me...