期刊论文

中文

中国组织工程研究

2095-4344

2013

17

46

8000-8003

10.3969/j.issn.2095-4344.2013.46.004

本校为其他机构

背景：目前人们对耳蜗的发育做了大量的研究工作，但主要集中在病理情况及发育畸形上，而对正常胚胎耳蜗发育过程及相关因子表达的研究较少。　　目的：观察转化生长因子β1在大鼠胚胎耳蜗中的表达情况。　　方法：取SD妊娠大鼠56只，从E9-E19逐日将孕鼠断颈，剖腹，迅速取出胚胎，解剖显微镜下修剪内耳标本，经脱水、脱钙、定向石蜡包埋、切片处理。苏木精-伊红染色后光镜下观察内耳形态学演变过程。免疫组织化学SABC法观察转化生长因子β1在胚胎耳蜗的表达。　　结果与结论：大鼠内耳的发育开始于E8的外胚层增厚区，E9就可观察到听泡及其周围间充质的出现，E9.5听囊及听骨链开始发育，E12.5蜗...

BACKGROUND:Many researches on the development of cochlea have been made, but mainly depend on the pathological conditions and developmental deformity, while the researches on the development process of normal embryonic cochlea and expression of related factors are rare. OBJECTIVE:To investigate the expression of transforming growth factorβ1 in rat embryonic cochlea. METHODS:Fifty-six Sprague Dawley rats were selected and the rats were pregnancy, and the embryos were obtained from the rats, trimmed the inner ear specimens under the anatomical m...