期刊论文

中文

营养学报

0512-7955

2007

29

2

146-149

10.3321/j.issn:0512-7955.2007.02.011

国家博士点基金（No.20020504008） 湖北省科技攻关项目（No.2003AA20412307）

本校为其他机构

目的:研究菹草类胡萝卜素提取物(carotenoids extracts from Potamogeton crispus L., CEPC)对人宫颈癌Hela细胞株增殖和凋亡的影响.方法:采用MTT法、流式细胞术(FCM)、荧光倒置显微镜和激光共聚焦显微镜(LSCM)观察等手段,研究不同浓度受试物对Hela细胞增殖抑制、细胞周期阻滞、细胞形态学变化及胞内~(2+)浓度变化的影响.结果:CEPC对Hela细胞增殖有明显的抑制作用;呈现G2/M期阻滞.荧光显微镜和LSCM观察到细胞核浓缩等典型的细胞凋亡形态特征.处理组细胞内Ca~(2+)浓度显著升高.结论:CEPC对Hela细胞有生长抑制作用,诱导细胞凋亡是这种作用的主要形式,胞内Ca2+浓度升高可能是细胞凋亡的作用机制之一...

Objective： To observe the effects of carotenoids extracts from Potamogoton crispus L. （CEPC） on proliferation and apoptosis of Hela cell line. Method： The cell growth inhibition was tested in MTT assay and cell cycle arrest was measured by flow cytometry （FCM）. Apoptotic cells were observed by fluorescent microscope and Laser scanning confocal microscopy （LSCM）. LSCM was also used to determine the Ca^2＋ concentration in cells. Results： CEPC had strong inhibition effects on the cell growth. The cell cycle progression was arrested at G2...