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2,4-dinitrotoluene (Dnt) perturbs yolk absorption, liver development and lipid metabolism/oxygen transport gene expression in zebrafish embryos and larvae

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成果类型:
期刊论文
作者:
Xiong, Jianglin;Sha, Hang;Zhou, Hualin;Peng, Lijuan;Wu, Lingying;...
通讯作者:
Wang, Rui
作者机构:
[Hu, Xianqin; Wu, Lingying; Xiong, Jianglin; Qiu, Yinsheng; Wang, Rui] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
[Sha, Hang] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.
[Zhou, Hualin] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China.
[Peng, Lijuan] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Wang, Rui] W
Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
语种:
英文
关键词:
2,4 dinitrotoluene;acyl coenzyme A oxidase;apolipoprotein A2;fatty acid binding protein;green fluorescent protein;heme oxygenase;hypoxia inducible factor 1alpha;microsomal triglyceride transfer protein;peroxisome proliferator activated receptor alpha;peroxisome proliferator activated receptor gamma;transferrin;2,4-dinitrotoluene;nitrobenzene derivative;oxygen;adult;animal experiment;animal model;animal tissue;Article;breathing;breeding;controlled study;down regulation;embryo;embryo development;fish breeding;gene expression;genetic transcription;larva;LC50;lipid metabolism;lipid transport;lipolysis;liver development;medical parameters;morphometry;mortality;nonhuman;oxygen transportation;polymerase chain reaction;post fertilization;real time polymerase chain reaction;reverse transcription polymerase chain reaction;RNA isolation;stereomicroscopy;upregulation;yolk absorption;zebra fish;animal;drug effect;embryology;gene expression regulation;larva;lipid metabolism;liver;metabolism;nonmammalian embryo;organogenesis;pharmacology;pollutant;toxicity;transport at the cellular level;Animals;Biological Transport;Dinitrobenzenes;Embryo, Nonmammalian;Embryonic Development;Environmental Pollutants;Gene Expression Regulation, Developmental;Larva;Lipid Metabolism;Lipolysis;Liver;Organogenesis;Oxygen;Zebrafish
期刊:
International Journal of Molecular Sciences
ISSN:
1661-6596
年:
2019
卷:
20
期:
15
页码:
3632
基金类别:
Funding: This work was supported by the National Natural Science Foundation of China (Grant No. 31201983).
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
动物科学与营养工程学院
摘要:
2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. The results showed that the 120-h post-fertilization LC50 of 2,4-DNT was 9.59 mg/L with a 95% confidence interval of 8.89–10.44 mg...

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