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Dramatic fluorescence enhancement of PCN-224 and its application in "turn off" immunoassay for sensitive detection of E. coli O157:H7 in milk.

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成果类型:
期刊论文
作者:
Fang, Bolong*;Liu, Xin;Peng, Juan;Li, Yuzhi;Gong, Zhiyong;...
通讯作者:
Fang, Bolong
作者机构:
[Gong, Zhiyong; Li, Yuzhi; Liu, Xin; Fang, Bolong] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
[Fang, Bolong] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: fangbl555@whpu.edu.cn
[Gong, Zhiyong; Li, Yuzhi; Liu, Xin] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China
[Lai, Weihua; Peng, Juan] State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
[Li, Yuzhi] Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan 430075, China
通讯机构:
[Fang, Bolong] K
Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address:
语种:
英文
关键词:
E. coli O157:H7;ELISA;Fluorescence enhancement;Fluorescence quenching;PCN-224
期刊:
Food Chemistry
ISSN:
0308-8146
年:
2024
卷:
445
页码:
138749
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
摘要:
In this study, a type of luminescent porous coordination network-224 (PCN-224) in alkaline conditions was synthesized with the dramatic fluorescence enhancement by 20.4 times, which was explained by the fact that the decrease of Zr(4+) content in alkaline conditions resulted in the partial recovery of the electron cloud density of 4,4',4'',4'''-(Porphine-5,10,15,20-tetrayl) tetrakis(benzoic acid) (TCPP). Given the large overlap between the excitation spectrum of PCN-224 and the absorption band of Ag nanoparticles (Ag NPs), the coating of the Ag...

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