期刊论文

中文

中国预防兽医学报

1008-0589

2009

31

8

637-641

(07CX024):武汉工业学院研究生教育创新项目

本校为第一机构

本实验研究低剂量鸡α-干扰素(ChIFN-α)对内鸡细胞免疫及细胞因子水平的影响.15日龄肉鸡连续混饮0.1 IU/mL、1 IU/mL和10 IU/mL剂量的ChIFN-α,分别在混饮后14 d和21 d翼静脉采取血样,采用中性红.结晶紫联合测定白细胞总数,经典方法进行白细胞分类计数,MTT比色分析法测定淋巴细胞转化能力,LDH释放法测定NK细胞的杀伤活性,双夹心ELISA法测定血清中IFN-γ、IL-4、IL-12水平.结果显示肉鸡连续混饮ChIFN-α后,能显著提高白细胞总数、淋巴细胞百分比和血清中IFN-γ水平,对IL-4、IL-12的浓度水平影响不显著,但提高IFN-γ/IL-4的比例,对淋巴细胞增殖和NK细胞的杀伤活性影响不明显.试验表明,混饮低剂量...

To determine the effects of low-doses of ChlFN-α on cell immunity and the level of Cytokines in broiler, fifteen day old broilers were given different doses of ChIFN-α（0.1 IU/mL, 1 IU/mL, 10 IU/mL） through drinking water for 21 days. Blood were collected at 14 d and 21 d, and leukocytes were monitored. The lymphocyte transformation capacity was detected by MTT colorimetric analysis, the NK cell activity of killing was measured by LDH release, and the IFN- γ, IL -4, 1L-12 level was detected by the double-sandwich ELISA method. The results s...