期刊论文

Study on the influence of miR-194b-3p on the adhesion ability of Streptococcus equi subsp. zooepidemicus to kidney epithelial cells of swine

中文

中国预防兽医学报

1008-0589

2017

39

12

1013-1016

10.3969/j.issn.1008-0589.201610004

湖北省自然科学基金（2014CFB344） 国家自然科学基金青年基金（31402036） 武汉市第三批“黄鹤英才（专项）计划”

本校为第一机构

microRNA分子在调节宿主细胞应对细菌感染的过程中起重要作用。为研究miR-194b-3p对马链球菌兽疫亚种(SeZ)粘附猪肾上皮细胞(PK15)能力的影响,本实验将miR-194b-3pagomir和antagomir分别转染PK15细胞,检测SeZ对PK15细胞粘附能力的变化。结果表明:在PK15细胞中转染miR-194b-3p agomir,SeZ C55138粘附率显著下降;转染miR-194b-3p antagomir, SeZ C55138粘附率显著上升,确定了miR-194b-3p能够显著抑制SeZC55138对PK15细胞的粘附。同时对其机制进行研究,显示PK15细胞表面CD44是miR-194b-3p的一个调控靶基因, miR-194b-3p通过调节CD44的表达参与影响了上述粘附过程。本实验为深入理解SeZ与PK15细胞的...

MicroRNAs are increasingly implicated in the host cell response to bacterial pathogens. In order to investigate whether miR-194b-3p regulates the adherence of Streptococcus equi subsp. zooepidemicus (SeZ) to porcine kidney cell line PK15, the miR-194b-3p agomir and antagomir were transfected into PK15 cells respectively and the adherence rate of SeZ to each was determined. The adherence rate of SeZ C55138 was significantly decreased when miR-194b-3p agomir was transfected into PK15, while the adherence rate was significantly increased when miR-1946-3p antagomir was transfected into PK15. These...