期刊论文

Purification and biochemical properties of cadmium binding protein from rice

中文

食品安全质量检测学报

2095-0381

2015

6

2269-2274

10.19812/j.cnki.jfsq11-5956/ts.2015.06.050

粮食公益性行业科研专项(2015449007) 武汉轻工大学重大项目培育专项(2011Z05) 武汉市国际合作项目(201231234466) 湖北省自然科学基金项目(2014CFB888) 武汉轻工大学研究生创新基金项目(2012cx012,2013cx011)~~

本校为第一机构

目的：研究大米镉结合蛋白(rice cadmium-binding protein, RCBP)的纯化及生化性质,探讨RCBP的形成机制。方法采用Sephedex G-75凝胶层析从乙醇沉淀的大米蛋白(Ethanol precipitation protein, EP)中纯化RCBP, SDS-PAGE鉴定RCBP的纯度及测定分子质量,氨基酸自动分析仪、FTIR分析RCBP的氨基酸组成及二级结构。结果凝胶层析纯化得到组分b(目标RCBP),组分b为纯度均一的RCBP,分子质量约为32 kDa。组分b中疏水性氨基酸占56.76%、芳香族氨基酸占19.41%,是一类富含疏水性氨基酸和芳香族氨基酸的镉结合蛋白。组分b中N-H相对于C=O以反式构型存在,二级结构只含有β-折叠和β-转角。结论镉通过疏水氨基酸或...

Objective To investigate the purification and biochemical properties of cadmium binding protein from rice and binding mechanism between cadmium and rice protein. Methods Rice cadmium-bining protein (RCBP) was purified from ethanol precipitation protein in rice using gel chromatography. The purity, molecular weight, amino acid composition, and secondary structure of RCBP were analyzed using SDS-PAGE, amino acid automatic analyzer, and FTIR. Results Fraction b (objective RCBP) with uniform purity and 32 kDa of molecular weight was collected from ...