期刊论文

中文

食品安全质量检测学报

2095-0381

2024

15

04

40-48

本校为第一机构

目的 建立一种快速、特异、灵敏地鉴定青鱼物种的方法.方法 以青鱼线粒体 16S rRNA基因的保守序列设计特异性 crRNA,并根据 crRNA 设计重组酶介导等温扩增(recombinase-mediated isothermal amplification,RAA)引物;建立青鱼的RAA-CRISPR/Cas12a快速鉴定方法,并对该方法的检测时间进行优化;同时,评估该方法的特异性和灵敏度,并对青鱼及其近缘物种的市售样品进行检测.结果 该方法仅需25 min完成检测;对青鱼物种的鉴定表现出显著的特异性,并对其近缘物种如草鱼、赤眼鳟、鳡鱼、倒刺鲃的检测结果均呈阴性;方法 灵敏度为 1.0×10‒3 ng/μL(以基因组DNA计);与DNA条形码技术相比,该方法在市售样品的检...

Objective To establish a method for rapid,specific and sensitive identification of Mylopharyngodon piceus species.Methods A specific crRNA was designed with the conserved sequence of Mylopharyngodon piceus mitochondrial 16S rRNA gene.Recombinase-mediated isothermal amplification(RAA)primers were designed based on the crRNA.A rapid identification method,RAA-CRISPR/Cas12a was developed for Mylopharyngodon piceus,and the detection time of this method was optimized.The specificity and sensitivity of the method were assessed,and commercially availab...