期刊论文

中文

微生物学通报

0253-2654

2023

50

7

2798-2811

10.13344/j.microbiol.china.220998

湖北省重点研发计划(2022BCA075) 武汉轻工大学科研项目(2022RZ027)。

本校为第一机构

【背景】聚γ-谷氨酸(poly-γ-glutamic acid, γ-PGA)是一种由芽孢杆菌代谢产生的同质氨基酸聚合物,在众多领域具有广泛的应用潜力。芽孢杆菌cwlO表达一种D,L-肽链内切酶,其对γ-PGA合成的影响...展开更多 【背景】聚γ-谷氨酸(poly-γ-glutamic acid, γ-PGA)是一种由芽孢杆菌代谢产生的同质氨基酸聚合物,在众多领域具有广泛的应用潜力。芽孢杆菌cwlO表达一种D,L-肽链内切酶,其对γ-PGA合成的影响机理尚不清晰。【目的】探究缺失cwlO对以不同前体发酵γ-PGA的影响及其机制。【方法】以地衣芽孢杆菌WX-02为出发菌株,构建缺失cwlO的重组菌。在3L发酵罐条件下对比重组菌和野生菌利用不同前体发酵...

[Background]Poly-γ-glutamic acid(γ-PGA),a homogeneous amino acid polymer produced by Bacillus,has application potential in a variety of fields.The cwlO in Bacillus can express a D,L-endopeptidase to influenceγ-PGA production,the underlying mechanism of which remains to ...MORE [Background]Poly-γ-glutamic acid(γ-PGA),a homogeneous amino acid polymer produced by Bacillus,has application potential in a variety of fields.The cwlO in Bacillus can express a D,L-endopeptidase to influenceγ-PGA production,the underlying mechanism of which remains...