The combination effects of acetaminophen and N-acetylcysteine on cytokines production and NF-κB activation of lipopolysaccharide-challenged piglet mononuclear phagocytes in vitro and in vivo
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30972213]; National Basic Research Program of ChinaNational Basic Research Program of China [2012CB126305]; Hubei Provincial Natural Science Foundation [2008CDA081]
机构署名：
本校为第一且通讯机构
院系归属：
生命科学与技术学院
动物科学与营养工程学院
摘要：
Lipopolysaccharide (LPS) is a known activator of mononuclear phagocytes. LPS activates the pro-inflammatory gene expression and induces the release of mediators/cytokines by TLR4-NF-kappa B signaling pathway. The purpose of this study was to investigate the effects of acetaminophen (AAP) and N-acetylcysteine (NAC), individually as well as in combination on LPS-induced cytokines production and NF-kappa B activation in piglets. AAP (0.125-1.0 mM) and NAC (0.0625-1.0 mM) down-regulate the expression of cytokines and inhibit NF-kappa B p65 protein transfer from the cytoplasm to the nucleus in vitr...
