Docosahexaenoic acid alleviates cell injury and improves barrier function by suppressing necroptosis signalling in TNF-α-challenged porcine intestinal epithelial cells
The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This study was supported by the Projects of Wuhan Science and Technology Bureau (2018020401011304), Innovative Research Groups of the Natural Science Foundation of Hubei Province (2019CFA015) and National Natural Science Foundation of China (31772615 and 31802070).
机构署名：
本校为第一且通讯机构
院系归属：
动物科学与营养工程学院
摘要：
Long-chain n-3 polyunsaturated fatty acids are known to have beneficial effects on intestinal health. However, the underling mechanisms are largely unknown. The present study was conducted to investigate whether docosahexaenoic acid (DHA) attenuates TNF-alpha-induced intestinal cell injury and barrier dysfunction by modulating necroptosis signalling. Intestinal porcine epithelial cell line 1 was cultured with or without 12.5 mu g/ml DHA, followed by exposure to 50 ng/ml TNF-alpha for indicated time periods. DHA restored cell viability and cell number triggered by TNF-alpha. DHA also improved b...
