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Differential proteome analysis along jejunal crypt-villus axis in piglets

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成果类型:
期刊论文
作者:
Xiong, Xia;Yang, Huansheng;Hu, Xihai;Wang, Xiaocheng;Li, Biao;...
通讯作者:
Yin, Yulong
作者机构:
[Li, Biao; Long, Lina; Xiong, Xia; Wang, Xiaocheng; Li, Tiejun; Yin, Yulong; Yang, Huansheng] Chinese Acad Sci, Inst Subtrop Agr,Res Ctr Hlth Breeding Livestock, Key Lab Agroecol Proc Subtrop Reg,Human Engn & Re, Minist Agr,Sci Observat & Expt Stn Anim Nutr & Fe, Changsha 410125, Hunan, Peoples R China.
[Yin, Yulong] Hunan Normal Univ, Sch Life Sci, Changsha 410008, Hunan, Peoples R China.
[Wang, Junjun; Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
[Hou, Yongqing; Wu, Guoyao; Yin, Yulong] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Yin, Yulong] C
Chinese Acad Sci, Res Ctr Hlth Breeding Livestock & Poultry, Changsha 410125, Hunan, Peoples R China.
Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
语种:
英文
关键词:
Proteomics;Small intestine;Suckling, piglet;Villus-crypt axis
期刊:
FRONTIERS IN BIOSCIENCE-LANDMARK
ISSN:
2768-6701
年:
2016
卷:
21
期:
2
页码:
343-363
DOI:
基金类别:
Xia Xiong and Huansheng Yang contributed equally to this work. The research reported in the present study was supported by National Natural Science Foundation of China (31330075; 31301988; 31110103909; 31472106), State Key Laboratory of Animal Nutrition (2004DA125184F1304), Knowledge Innovation Program of Institute of Subtropical Agriculture, The Chinese Academy of Sciences (ISACXLYQY-QN-1205), the Natural Science Foundation of Hubei Province (no. 2013CFA097 and 2013CFB325), the Hubei Hundred Talent program, and Texas A&M AgriLife Research (H-8200).
机构署名:
本校为其他机构
院系归属:
动物科学与营养工程学院
摘要:
The brush-border membrane (BBM) of enterocytes is responsible for the digestion and absorption of nutrients and ions in the small intestine. To identify the BBM proteins involved in epithelial cell maturation along the crypt-villus axis, enterocytes were sequentially isolated from the villus tip to the crypt of the jejunum from 21-day-old suckling piglets. After preparation of BBM vesicles, we detected 194 proteins in the jejunal epithelial cells by isobaric tags using relative and absolute quantification (iTRAQ) techniques. Of these, 56 BBM proteins were differentially expressed along the cry...

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