Enabling Efficient Genetic Manipulations in a Rare Actinomycete Pseudonocardia alni Shahu

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成果类型：

期刊论文

作者：

Li, Jie;Wang, Baiyang;Yang, Qing;Si, Han;Zhao, Yuting;...

通讯作者：

Zheng, Yanli;Peng, WF;Zheng, YL

作者机构：

[Zhao, Yuting; Zheng, Yanli; Peng, Wenfang; Wang, Baiyang; Si, Han; Li, Jie; Peng, WF; Yang, Qing] Hubei Univ, Hubei Engn Res Ctr Bioenzyme Catalysis, Environm Microbial Technol Ctr Hubei Prov, Sch Life Sci,State Key Lab Biocatalysis & Enzyme E, Wuhan, Peoples R China.

[Li, Jie; Zheng, Yanli] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan, Peoples R China.

[Wang, Baiyang] Wuhan Univ, Coll Life Sci, Dept Microbiol, Wuhan, Peoples R China.

通讯机构：

[Zheng, YL; Peng, WF ] H

[Zheng, YL ] W

Hubei Univ, Hubei Engn Res Ctr Bioenzyme Catalysis, Environm Microbial Technol Ctr Hubei Prov, Sch Life Sci,State Key Lab Biocatalysis & Enzyme E, Wuhan, Peoples R China.

Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan, Peoples R China.

语种：

英文

关键词：

Pseudonocardia;Genetic Manipulation;Conjugal transfer;Gene integration;Gene Deletion

期刊：

Frontiers in Microbiology

ISSN：

1664-302X

年：

2022

卷：

页码：

848964

DOI：

10.3389/fmicb.2022.848964

基金类别：

This work was supported by the Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering (SKLBEE2021019), and the Innovation Base for Introducing Talents of Discipline of Hubei Province (2019BJH021). YZe and WP acknowledge the support from the State Key Laboratory of Biocatalysis and Enzyme Engineering.

机构署名：

本校为通讯机构

院系归属：

生命科学与技术学院

摘要：

Pseudonocardia species are emerging as important microorganisms of global concern with unique and increasingly significant ecological roles and represent a prominent source of bioactive natural products, but genetic engineering of these organisms for biotechnological applications is greatly hindered due to the limitation of efficient genetic manipulation tools. In this regard, we report here the establishment of an efficient genetic manipulation system for a newly isolated strain, Pseudonocardia alni Shahu, based on plasmid conjugal transfer from Escherichia coli to Pseudonocardia. Conjugants ...

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Enabling Efficient Genetic Manipulations in a Rare Actinomycete Pseudonocardia alni Shahu

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