Hubei Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [2020CFB803]; China Ocean Mineral Resources Research and Development Association [DY135-B2-06]
机构署名：
本校为第一且通讯机构
院系归属：
生命科学与技术学院
摘要：
Mannanases catalyze the cleavage of β-1,4-mannosidic linkages in mannans and have various applications in different biotechnological industries. In this study, a new β-mannanase from Verrucomicrobiae DG1235 (ManDG1235) was biochemically characterized and its enzymatic properties were revealed. Amino acid alignment indicated that ManDG1235 belonged to glycoside hydrolase family 26 and shared a low amino acid sequence identity to reported β-mannanases (up to 50% for CjMan26C from Cellvibrio japonicus). ManDG1235 was expressed in Escherichia co...
