期刊论文

中文

食品科学

1002-6630

2005

26

5

211-216

10.3321/j.issn:1002-6630.2005.05.047

教育部博士点基金资助项目（20020504008）； 湖北省科技攻关计划项目（2003AA204B07）；

本校为其他机构

采食菹草的蛋鸭所产红心蛋蛋黄的类胡萝卜素提取物具有良好的抑制S180肿瘤的作用,为阐明其抗肿瘤作用机制,研究了菹草类胡萝卜素提取物(CEPC)对人肝癌细胞QGY-7703细胞增殖和凋亡的影响,本文报道采用体外培养、细胞增殖实验,然后以可见光、荧光、激光扫描共聚焦显微镜(LSCM)观察肝癌细胞的形态变化.结果表明:分别用含10、20和40μmol/L的CEPC的培养介质处理QGY-7703细胞24、48和72h,在可见光、荧光显微镜、LSCM下观察细胞形态,分别出现了肝癌细胞数量明显减少、皱缩变形、体积缩小、触角伸长、细胞表面凸起多个小泡,显示亮黄色荧光的细胞核呈现"新月状"、条状甚至碎片状形状,细胞核中呈黄色的DN...

The carotenoids extracted from red-yolk eggs of ducks fed on Potamogoton crispus L.had a marked inhibition on the proliferation of S 180 tumor. The effects of carotenoids extracts from Potamogoton crispus L. (CEPC) on proliferation and apoptosis of human hepatoma cells QGY-7703 were studied so as to probe the anti-tumor mechanism of the red-yolk eggs. This paper adopted visible light, fluorescence and Laser Scanning Confocal Microscope (LSCM) to observe the cellular morphology of hepatoma cells under the influence of CEPC. After cells were trea...