期刊论文

中文

食品科学

1002-6630

2008

29

10

80-84

10.3321/j.issn:1002-6630.2008.10.011

湖北省稻谷加工工程技术研究中心基金项目（2007016）；

本校为第一机构

采用聚酰胺柱层析和乙醇多次沉淀的方法分离纯化,获得燕麦β-葡聚糖主要组分Oglu-1,经HPLC、UV、IR、1~HNMR和((13)~CNMR证实Oglu-1为β-D-均聚线型葡聚糖,糖基之间通过β-D-(1→4)和(1→3)糖苷键连接,二者之比为2.1:1,激光光散射测定多糖的相对分子质量为4.49×105。通过测定特性黏度和激光光散射分析建立了Oglu-1水溶液中的Mark-Houwink方程([η]=0.161Mw~(0.585)和均方根旋转半径-相对分子质量关系模型,证实了Oglu-1分子在溶液中的形态为柔顺性线型高分子链;利用AFM直接观测到Oglu-1是分子链长度约250nm,宽度约为5～10nm,高2～3nm的无支链线型柔顺高分子;CD分析表明,多糖分子量、多糖浓度及p...

Oglu-1, a main constituent of Oglu, was obtained by multiple ethond precipitation and column chromatography. It was found that Oglu-1 is composed of glucose units only and joined by β-D-（1→~4） and β-D-（1→3） linkage with the linkage number ration 2.1：1 by using the methods of UV, IR, ^1HNMR,^13CNMR and HLPC. The average molecular weight of Oglu-1 is 4.49 × 10^5 determined by GPC-LLS. The Oglu-1 is semi-flexible chain,which was proved by the Mark-Houwink equation （[ η ]=0. 161Mw^0.585） and the relation model of Mw-〈S^2〉^1/2. The mo...