Zein was prepared by organic solvent extraction. The change of ethanol concentration was measured by alcoholmeter before and after absorption ofzein. The single factor analysis was adapted to determining the optiomal conditions such as pH, temperature, ethanol concentration and ratio of liquid to solid. The results showed that there is the maximum ethanol adsorption under the conditions of ethanol concentration 30%, ratio of liquid to solid 1:10, 20 ℃ and pH 6. The results lay a ...