期刊论文

中文

食品科学

1002-6630

2009

30

18

25-28

10.3321/j.issn:1002-6630.2009.18.001

基金项目：国家“863”计划重大项目（2006AA092444 2007AA091802） 省部产学研结合项目（2007A090302059）

本校为第一机构

采用大孔吸附树脂对碱性蛋白酶水解、水解度为34.52%的鱼降压肽进行分离。结果表明,DA201-C型大孔吸附树脂对鱼降压肽吸附性最好,乙醇体积分数为75%时洗脱下来的组分具有最高的ACE抑制活性,其IC50为1.52mg/ml。鱼降压肽中灰分由分离前的9.47%减少到分离后的2.34%,肽和蛋白质含量分别由分离前的72.81%和81.26%增加到分离后的80.24%和92.42%。活性稳定性分析显示,胃蛋白酶和胰酶、中性和低酸性pH值、55℃以下温度以及1000mmol/L以内的NaCl浓度对鱼降压肽的ACE抑制活性影响较小。

Various macroporous adsorption resins were used for the isolation of antihypertensive peptides from grass carp protein hydrolysate with the hydrolysis degree of 34.52% and the stability of angiotensin-converting enzyme （ACE） inhibitory activity of the peptides was analyzed. Among 8 types of macroporous adsorption resin tested, DA201-C had the best adsorption capability. The peptide fraction eluted by 75% ethanol displayed the highest ACE inhibitory activity with the IC50 of 1.52 mg/ml. After isolation, the ash content was decreased from 9.47%...