期刊论文

中文

食品科学

1002-6630

2011

32

12

169-173

(2010AA023003):国家"863"计划重点项目 (Q20091808):湖北省教育厅中青年人才项目 (08cx021):武汉工业学院研究生创新基金项目

本校为第一机构

为建立大米肽含量的快速测定方法,选择三氯乙酸(TCA)、乙醇作为沉淀剂分级大米肽中高分子质量的蛋白质,研究TCA、乙醇浓度和体积以及温度和时间对沉淀效果的影响。结果表明:TCA沉淀法的较佳条件为TCA质量浓度150g/L、TCA与大米肽体积比3:1、35℃静置10min,测定大米肽含量为(54.2±0.6)%;乙醇沉淀法的较佳条件为乙醇体积分数75%、乙醇与大米肽体积比5:1、25℃静置10min,测定大米肽含量为(50.6±0.1)%;在30℃条件下反应30min显色结果较稳定。乙醇沉淀法与TCA沉淀法相比线性范围广、灵敏度高、重复性好。

In order to establish a rapid sample pretreatment method for spectrometrically（based on Biuret reaction） determining peptides in rice hydrolysate prepared with alcalase,trichloroacetic acid（TCA） and ethanol were separately used as precipitants to remove high molecular weight proteins from the hydrolysate and three conditions including precipitant dosage and concentration,temperature and standing time were examined.The optimal conditions for TCA precipitation were adding a 3-fold volume of 150 g/L TCA solution to the hydrolysate and then sta...