期刊论文

中文

食品科学

1002-6630

2012

33

15

34-37

国家“863”计划项目（2010AA023003） 武汉工业学院校研究生创新基金项目（2011cx013）

本校为第一机构

以草鱼为原料制取生理活性肽,采用高效液相色谱法(HPLC)测定其对血管紧张素转化酶(ACE)的抑制活性,采用DPPH自由基清除法、氧自由基清除能力(ORAC)法研究其抗氧化性。结果表明:草鱼肽抑制ACE活性IC_(50)为0.229mg/mL,清除DPPH自由基的IC_(50)为7.630mg/mL,ORAC值为1317.962μmol Trolox/g,采用草鱼制备有效的生理活性肽是可行的。

In this study,bioactive peptides were prepared from grass carp meat by enzymatic hydrolysis and ultrafiltration.Their angiotensin I-converting enzyme（ACE） inhibitory activity was determined by high performance liquid chromatography（HPLC） and antioxidant activity was evaluated by DPPH radical scavenging and ORAC（oxygen radical absorbance capacity） assays.The results showed that the prepared bioactive peptides had strong ACE inhibitory activity with IC50of 0.229 mg/mL.The concentration needed to scavenge 50% DPPH radicals was 7.630 mg/mL an...