In this study, 69 thermotolerant yeast strains were isolated from 29 different soil samples, and the fermentation performance of these strains at high temperature was also detected. Based on 26S rDNA D1/D2 domain sequence analysis and physiological characterization, these strains were phylogenetically related to 7 species in 6 genera, including Candida tropicalis (39.1% of the total yeast strains), K. marxianus (23.4%), I. orientalis (29.7%), M. guilliermondii (1.6%), K. bovina (1.6%), C. palmioleophila (1.6%) and S. cerevisiae (3.1%). C. tropicalis were the most widely distributed species. Th...