期刊论文

Zhou, Guoping(wjczgp@163.com)

中文

食品科学

1002-6630

2016

37

22

205-209

10.7506/spkx1002-6630-201622031

武汉轻工大学大学生科研项目

本校为第一机构

对某番茄酱生产企业提供的辐照杀菌(辐照剂量≥15 kGy)的5 L无菌袋进行菌落计数、分离和鉴定。3 L无菌水反复漂洗,滤膜过滤后进行平板菌落计数,分离纯化后采用16S rDNA和dnaJ序列分析分别鉴定到属和种。结果表明,5批次的样品中均有细菌检出,数量在5~132 CFU/袋,9个典型菌落经过16S rDNA序列分析后,分别属6个属,其中栖水菌属(Enhydrobacter)、不动杆菌属(Acinetobacter)和考克斯菌属(Kocuria)各1株,微球菌属(Micrococcus)、微杆菌属(Microbacterium)和芽孢杆菌属(Bacillus)各2株;dnaJ序列分析则将一株葡萄球菌C8-3鉴定到种-表皮葡萄球菌(Staphylococcus epidermidis)。辐照杀菌的无菌袋虽然微生物...

Objective: To count, isolate and identify the radiation-resistant microorganisms present in 5 L sterile pouches provided by a tomato paste producer, sterilized by radiation at doses equal to or greater than 15 kGy. Methods: The pouches were washed repeatedly with 3 L of sterile water. The water was filtrated through a membrane and then spread on medium plates for counting microbial colonies. Finally, the microorganisms were isolated, purified and identified at the genus and species levels by analysis of their 16S rDNA sequences and dnaJ sequences. Results: All 5 batches of samples were found t...