期刊论文

Zhou, Guoping(wjczgp@163.com)

中文

食品科学

1002-6630

2016

37

20

198-202

10.7506/spkx1002-6630-201620034

2015—2016武汉轻工大学大学生创新创业训练计划项目（CXXL20151004）；

本校为第一机构

目的:检测15个批次浓缩果汁中的耐热霉菌并对其进行鉴定。方法:80 ℃、30 min热处理后涂布平板菌落计数法分离和计数浓缩果汁中的耐热霉菌,分离纯化后观察其显微形态和菌落形态,再采用28S rDNA序列分析鉴定霉菌的种属。结果:这15批次浓缩果汁中有8个样品检出耐热霉菌,检出率达53.3%;检出的耐热霉菌以正青霉(Eupenicillium)和篮状菌(Talaromyces)为主,还有少量白耙齿菌、栓菌和座囊菌属。结论:进口或国产浓缩果汁都存在耐热霉菌污染,其中尤其值得注意的是检出了既耐热又能产生真菌毒素的正青霉和篮状菌。

Objective: To detect and identify the heat resistant molds in 15 batches of concentrated fruit juice. Methods: After heat treatment at 80℃for 30 min to kill the heat-sensitive fungi and activate the heat-resistant spores, we spread the concentrated juices on medium plates and counted the fungal colonies after 14-21 d culture. Secondly, the fungi were purified, observed and identified by 28S rDNA sequence analysis. Results: Heat resistant fungi were detected in eight out of 15 batches (53.3%) of concentrated fruit juice, consisting mainly of Eupenicillium and Talaromyces, together with a ...