期刊论文

Detection of Sulforaphene Content in Radish Seed Oil and Influence of Processing Conditions on Sulforaphene Content

中文

食品科学

1002-6630

2020

41

20

198-204

10.7506/spkx1002-6630-20191015-142

农产品加工与转化湖北省重点实验室开放课题(2018HBSQGDKF04) 国家自然科学基金青年科学基金项目(31601505) 国家自然科学基金面上项目(31371783)。

本校为第一机构

通过对萝卜籽毛油样品前处理萃取方式的优化和加标回收实验,确定采用两步溶剂萃取方式检测萝卜籽油中莱菔素（sulforaphene,SFE）含量。考察萝卜籽预处理对SFE生成量的影响,以毛油中SFE含量为指标,分别用丙酮、二氯甲烷、乙醚、正己烷对其进行浸出法取油,并对比常规精炼与无水常温精炼工艺对油中SFE含量的影响。结果表明:用水和二氯甲烷进行两步萃取方式检测的萝卜籽毛油峰形好,响应值高,回收率和重复性较好。以SFE含量为指标,最佳的水分调节方式和预处理工艺是将萝卜籽进行粉碎后,直接喷洒调节水分质量分数到8%,25℃酶解10 min。最佳浸出溶剂为丙酮,浸出毛油中SFE含量高达832.36 mg/kg。采用常...

Through optimization of sample pretreatment conditions as well as spiked recovery experiments,a two-step extraction method was proposed to detect the sulforaphene(SFE)content in radish seed oil.The effect of radish seed pretreatment on SFE production was investigated.Acetone,dichloromethane,diethyl ether,and n-hexane were separately used to extract crude oil from pretreated radish seeds,in order to compare the effects of conventional refining and anhydrous room temperature refining on the retention rate of SFE in the oil.The results indicated t...