期刊论文

He XinYi

英文

科学通报(英文版)

2095-9273

2013

58

8

864-868

10.1007/s11434-012-5554-8

The authors are grateful to Dr. Tobias Kieser for valuable discussions. This work was supported by the National Natural Science Foundation of China (31170083), the Ministry of Science and Technology (2012CB721004), and the Shanghai Municipal Council of Science and Technology．H H Xu acknowledges support of Wang Family Faculty Fellowship from California State University.

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5-Hydroxymethylcytosine (5hmC) was present in T-even phage and mammalian DNA. 5hmC in phage is formed by hydroxymethylation of the cytosine base in deoxycytidylate (dCMP) by deoxycytidylate hydroxymethylase (CH), which uses the solvent water as the hydroxyl group donor. By contrast, 5hmC is formed in mammal zygotes by the oxidation of 5-methylcytosine (5mC). 5hmC was also present in a nucleoside antibiotic mildiomycin and its formation is governed by a cytidylate hydroxymethylase MilA. However, the catalytic mechanism remains unknown. In the pr...

5-Hydroxymethylcytosine (5hmC )T 甚至是在场的在里面噬菌体和哺乳动物的 DNA。在噬菌体的 5hmC 被 deoxycytidylate hydroxymethylase (CH ) 被 cytosine 底的 hydroxymethylation 在 deoxycytidylate (dCMP ) 形成，它把溶剂水用作氢氧根组施主。由对比， 5hmC 被 5-methylcytosine (5mC ) 的氧化在哺乳动物接合子形成。5hmC 也是在 nucleoside 抗菌素 mildiomycin 的现在，它的形成被 cytidylate hydroxymethylase MilA 管理。然而，催化机制仍然保持未知。在现在的学习，我们净化了他的标注 MilA 并且喂它的在里面有 H2 18O 的 vitro 反应。产品的最小公倍数分析表明 18O 被合并到 hydrox...