期刊论文

Effect of thermal denaturation on the properties of collagen sponges from fish

Wang, Haibo

中文

功能材料

1001-9731

2015

46

9

09045-09051

10.3969/j.issn.1001-9731.2015.09.009

国家自然科学基金面上资助项目（21376183） 武汉市科技局应用基础研究资助项目（2013020501010177） 湖北省高等学校优秀中青年科技创新团队计划资助项目（T201208）

本校为第一且通讯机构

以草鱼皮为原料提取天然胶原蛋白,经DSC、圆二色谱和SDS-PAGE分析证实为具有完整三螺旋分子结构的Ⅰ型胶原。在不同条件下对该胶原进行热处理后分别获得了低变性、高变性和完全变性的胶原样品,热变性焓测定结果表明热变性程度分别为16.9%,57.9%和100%。在此基础上,重点研究了热变性处理后胶原结构和胶原海绵材料性能的变化。结果表明,草鱼皮胶原变性的起始温度(T_o,33 ℃)是其分子结构和胶原海绵材料性能改变的阀值温度。高于该温度条件下的短暂受热历史即可导致胶原三螺旋结构的部分或完全解离。同时,胶原变性程度的增加,材料结构的有序性、材料力学性能和耐酶解能力也随之降低,但是促细胞增殖...

In this paper, native collagen (NC) was extracted from skin of grass carp, and it was proved type I collagen with intact triple helix structure via the DSC and circular dichroism and SDS-PAGE analysis. Low thermal denaturation (LD), high thermal denaturation (HD) and complete thermal denaturation(CD)collagen samples were obtained by heat treatment of NC under different conditions, and the degrees of thermal denaturation of them were 16.9%, 57.9 and 100% respectively, according to the changes of thermal denaturation enthalpy from DSC determination. On this basis, the research focused on the inf...