期刊论文

Xiao, XJ;Mao, ZH;Li, LJ

英文

Analytica Chimica Acta

0003-2670

2023

1280

341821

10.1016/j.aca.2023.341821

National Natural Science Foundation of China [82260290]; sixth batch of young scientific and technological talent support projects in Ningxia Hui Autonomous Region; National Key Research and Development Program of China [2021YFC2701402]; Anhui Province Key Laboratory of Reproductive Health and Genetic; Anhui Medical University; Research Plan Project of Hunan Provincial Health Commission [202205033867, 202305036567]; Hunan Provincial Science and Technology Innovation Plan [2021SK53207, 2021SK53204]

本校为通讯机构

DNA integrity is crucial for the clinical pregnancy outcome and offspring health, while detection methods currently used (comet assay, TUNNEL assay, SCSA, etc.) can only provide the ratio of positive sperms at the cellular level and are unable to quantitatively detect the breakpoints at the DNA molecular level. Herein, we developed a detection system based on terminal deoxynucleotidyl transferase and DNA strand displacement fluorescent probe, which could efficiently and conveniently measure the number of 3'-OH (equivalent to the number of breakpoints). We further investigated the use of this t...