Binding properties of oxytetracycline (OTC) and human serum albumin (HSA) have been studied in details. This investigation was based on flow injection chemiluminescence (FI-CL) analysis and ultrafiltration (UF) sampling technique. Chemiluminescence (CL) emission was observed during oxidation or OTC with KMnO4 in acidic medium in the presence of formaldehyde as a sensitizer. Coupled with flow injection technique, OTC can be determined in the range of 8.0 x 10(-7)-1.2 x 10(-4) mol L-1. Oxytetracycline and HSA were mixed in different molar ratios and incubated at 37+/-0.5 degrees C. An ultrafiltr...