期刊论文

中文

肿瘤防治研究

1000-8578

2011

38

7

740-744

10.3971/j.Issn.1000-8578.2011.07.003

广东医学院博士启动基金资助项目（B2009011） 广东省医学科学技术研究基金资助项目（B2010247）

本校为其他机构

目的 研究在不同叶酸偶联率下,叶酸-壳聚糖纳米粒对宫颈癌细胞系HeLa凋亡的影响.方法 还原胺法合成不同叶酸偶联率的叶酸-壳聚糖复合物,紫外分光光度仪测定叶酸偶联率.将复合物包裹绿色荧光蛋白表达基因,电子显微镜观察纳米粒形态及大小.转染HeLa细胞7d后流式细胞仪测定转染效率及凋亡率.结果 通过改变叶酸及壳聚糖不同的反应比,共合成四种不同偶联率的叶酸-壳聚糖复合物,偶联率依次是3%、7.5%、11.2%和17%.透射电子显微镜显示叶酸-壳聚糖-DNA纳米粒为近似球型,表面光滑、均质的纳米粒.上述纳米粒粒径分别为(289.6±0.7)nm、(78.1±0.3)nm、(186.6±0.6)nm和(212.2±0.5)nm.转染7d后叶酸偶联率为...

Objective To study the effect of folic acid coupling rate on anti-tumor property of folic acid-chitosan nanoparticles.Methods Folate-chitosan complexes at different folic acid coupling rate were synthesized by using a reductive amidation reaction.The green fluorescent protein expression gene was encapsulated with complexes.The shape and size of nanoparticles were detected by electron microscope.The transfection efficiency and cell apoptosis rate were evaluated through the flow cytometer.Results The nanoparticles presented the different sizes of...