Immunochromatographic Strip Based on Tetrahedral DNA Immunoprobe for the Detection of Aflatoxin B1 in Rice Bran Oil

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成果类型：

期刊论文

作者：

Xu, Lin;Qu, Wenli;Hao, Xiaotong;Fang, Min;Yang, Qing;...

通讯作者：

Xu, L;Li, PW

作者机构：

[Qu, Wenli; Hao, Xiaotong; Gong, Zhiyong; Xu, Lin; Li, Yuzhi; Fang, Min; Yang, Qing] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

[Gong, Zhiyong; Xu, Lin; Fang, Min; Yang, Qing] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.

[Gong, Zhiyong; Xu, Lin; Fang, Min; Yang, Qing] Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

[Li, Yuzhi] Hubei Prov Inst Food Supervis & Test, Key Lab Detect Technol Focus Chem Hazards Anim Der, Wuhan 430075, Peoples R China.

[Li, Peiwu; Li, PW] Chinese Acad Agr Sci, Oil Crops Res Inst,Minist Agr & Rural Affairs, Key Lab Detect Mycotoxins,Qual Inspect & Test Ctr, Natl Reference Lab Agr Testing Biotoxin,Lab Qual &, Wuhan 430062, Peoples R China.

通讯机构：

[Xu, L ] W

[Li, PW ] C

Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.

Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

语种：

英文

关键词：

aflatoxin B1;immunochromatographic assay;immunoprobe;tetrahedral DNA nanostructure

期刊：

Foods

ISSN：

2304-8158

年：

2024

卷：

期：

页码：

2410-

DOI：

10.3390/foods13152410

基金类别：

Conceptualization, L.X. and P.L.; funding acquisition, L.X.; investigation, W.Q. and X.H.; methodology, L.X. and M.F.; project administration, Q.Y. and Z.G.; supervision, Z.G.; writing—original draft, L.X. and W.Q.; writing—review a editing, L.X., Q.Y. and Y.L. All authors have read and agreed to the published version of the manuscript. This research was funded by the National Natural Science Foundation of China (32102072), Wuhan Knowledge Innovation Project (2022020801020399).

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

摘要：

Aflatoxin B1 (AFB1), a widespread contaminant in food and feeds, poses a threat to the health of animals and humans. Consequently, it is significant to develop a rapid, precise and highly sensitive analytical method for the detection of AFB1. Herein, we developed an immunochromatographic strip (ICS) based on a tetrahedral DNA (TDN) immunoprobe for AFB1 determination in rice bran oil. Three sizes of TDN immunoprobes (AuNP-TDN13bp-mAb, AuNP-TDN17bp-mAb, AuNP-TDN26bp-mAb) were constructed, and the performance of these three immunoprobes, including the effective antibody labeling density and immun...

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Immunochromatographic Strip Based on Tetrahedral DNA Immunoprobe for the Detection of Aflatoxin B1 in Rice Bran Oil

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