期刊论文

中文

中国临床药理学杂志

1001-6821

2014

30

3

204-207

10.13699/j.cnki.1001-6821.2014.03.023

武汉轻工大学研究生创新基金资助项目（2012cx021）；

本校为第一机构

目的研究银杏内酯B 注射液对大鼠体外肝微粒体细胞色素P450 1A2 (CYP1A2)酶活性的影响。方法用大鼠体外肝微粒体孵育法,以非那西丁为 CYP1A2的探针药物,HPLC 法测定非那西丁代谢物对乙酰氨基酚浓度,比较不同剂量(0. 6,6, 60, 150 μg·L~(-1))银杏内酯B注射液组与空白组间非那西丁代谢率(对乙酰氨基酚浓度/非那西丁浓度)的差异。结果4个剂量银杏内酯B注射液组的非那西丁代谢率分别为(11. 03 ± 0. 39)%,(11. 75 ± 0. 22)%, (11. 08 ± 0. 70)%及(11. 59 ± 0. 30)%,空白组为(11. 32 ± 0. 37)%,各剂量组与空白组比较均差异无统计学意义(P > 0. 05)。结论银杏内酯B 注射液对大鼠体外肝微粒体CYP...

Objective To investigate the effects of ginkgolide B （GB） injection on the activity of liver microsome cytochrome P450 1A2 of rats in vitro. Methods Liver microsomes of male rats were prepared and incubated with GB injection at the presence of the substrate probe of phenacetin in vitro. The concentrations of the metabolite acetaminophen were determined by HPLC. The effects of GB injections on the activity of CYP1A2 were evaluated by comparing the difference of probe drug metabolic rates between different GB injection dose groups （0.6, rates ...