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Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-Phthalaldehyde

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成果类型:
期刊论文
作者:
Hou, Yongqing;Li, Xilong;Dai, Zhaolai;Wu, Zhenlong;Bazer, Fuller W;...
通讯作者:
Wu, G.
作者机构:
[Hou, Yongqing] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China
[Li, Xilong] Institute of Feed Science, The Chinese Academy of Agricultural Sciences, Beijing, 100081, China
[Dai, Zhaolai; Wu, Zhenlong] State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China
[Bazer, Fuller W] Department of Animal Science, Texas A&M University, 2471 TAMU, RM 133 Kleberg, College Station, TX, 77843-2471, USA
[Wu, Guoyao] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, China. g-wu@tamu.edu
通讯机构:
Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, China
语种:
英文
关键词:
*Cysteine;*Glutathione;*HPLC;*Polyamines;*o-Phthalaldehyde
期刊:
Methods in Molecular Biology
ISSN:
1064-3745
年:
2018
卷:
1694
页码:
105-115
机构署名:
本校为第一且通讯机构
院系归属:
动物科学与营养工程学院
摘要:
Glutathione (GSH) forms conjugates with polyamines in prokaryotes and eukaryotes. There is also evidence suggesting cross-talk between GSH and polyamines to regulate cellular homeostasis and function, particularly under the conditions of oxidative stress. Because of its versatile roles in cell metabolism and function, a number of high performance liquid chromatography (HPLC) methods have been developed for glutathione analysis. Here, we describe our rapid and sensitive method for the analysis of GSH and the oxidized form of glutathione (GS-SG) ...

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