Electrochemical immunosensor based on antibody-oriented probe for the detection of AFB1 in rice bran oil

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成果类型：

期刊论文

作者：

Xu, Lin*;Hao, Xiaotong;Chen, Li;Qu, Wenli;Duan, Shuo;...

通讯作者：

Xu, Lin;Dai, H

作者机构：

[Dai, Huang; Qu, Wenli; Wu, Jine; Hao, Xiaotong; Gong, Zhiyong; Duan, Shuo; Xu, Lin; Wang, Qiao; Yang, Qing] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

[Wu, Jine; Gong, Zhiyong; Duan, Shuo; Xu, Lin; Wang, Qiao; Dai, Huang; Yang, Qing] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.

[Wu, Jine; Gong, Zhiyong; Duan, Shuo; Xu, Lin; Wang, Qiao; Dai, Huang; Yang, Qing] Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

[Chen, Li] Hubei Prov Inst Food Supervis & Test, Wuhan 430075, Peoples R China.

[Chen, Li] Key Lab Detect Technol Focus Chem Hazards Anim Der, Wuhan 430075, Peoples R China.

通讯机构：

[Dai, H ; Xu, L] W

Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

语种：

英文

关键词：

Aflatoxin B 1;Electrochemical immunosensor;Oriented antibody;Rice bran oil

期刊：

Journal of Food Composition and Analysis

ISSN：

0889-1575

年：

2024

卷：

130

页码：

106198

DOI：

10.1016/j.jfca.2024.106198

基金类别：

This work was supported by the Wuhan Knowledge Innovation Project (2022020801020399), the National Natural Science Foundation of China (32102072) and the Key Research and Development Projects in Hubei Province, China (2021BCA113)

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

摘要：

Aflatoxin B1 is one of the most toxic mycotoxins, posing a serious threat to human health. There is an urgent need to develop sensitive, rapid and selective ways for the detection of AFB1. Herein, an electrochemical immunosensor was fabricated using the antibody-oriented probe (gold nanoparticle-Streptococcal protein A-anti-AFB1 monoclonal antibody, AuNP-SPA-mAb) to analyze AFB1 in rice bran oil. AuNP-SPA-mAb probe was constructed by the introduction of streptococcal protein A to ensure the controlled immobilization of antibody. AFB1 competed w...

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Electrochemical immunosensor based on antibody-oriented probe for the detection of AFB1 in rice bran oil

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