期刊论文

Functional verification of vimentin as an alarmin

中文

中国免疫学杂志

1000-484X

2016

32

6

798-802

10.3969/j.issn.1000-484X.2016.06.006

湖北省自然科学基金项目（2015CFC845） 国家自然科学基金项目（31370867） 武汉轻工大学研究生创新基金项目（2013CX021）资助

本校为第一机构

目的:鉴定胞外可溶性波形蛋白(Vim)促进相关细胞增殖、活化、趋化的能力。方法:体外检测Vim刺激大鼠脾细胞的增殖情况。体内检测Vim免疫小鼠的外周血淋巴细胞数及Vim抗体水平。收集小鼠腹腔巨噬细胞与不同浓度Vim共培养,检测其对巨噬细胞吞噬鸡红细胞能力的影响。以Transwell小室法检测Vim对NIH3T3成纤维细胞的趋化作用。结果:体外实验Vim剂量依赖性地促进大鼠脾细胞增殖,16 μg /ml浓度的Vim刺激预致敏或未致敏脾细胞增殖率分别高达(196. 0±9. 7) %、(208. 9±4. 6) %,且该二者无显著性差异。体内实验单用Vim免疫小鼠的外周血淋巴细胞浓度(109 L-1)激增(5. 74±0. 51 vs. 1. 69±0. 13) ,同时...

Objective：To identify the function of extracellular soluble vimentin that promotes proliferation, activation and chemotaxis of inflammatory cells. Methods： The proliferation of rat splenocytes stimulated with vimentin was evaluated in vitro. The lymphocyte counts and vimentin-antibody levels of peripheral blood in mice immunized with vimentin were detected in vivo. Peritoneal macrophages were collected and cultured with different concentrations of vimentin to detect the effect on phagocytosing chicken red blood cells. The chemotaxis of NIH3T3...