期刊论文

Determination of Clindamycin Phosphate in Compound Sodium Phenytoin Gels by HPLC

中文

中国药师

1008-049X

2015

4

690-691

10.3969/j.issn.1008-049X.2015.04.058

2011年湖北省教育厅重点科研项目（编号:D20112006）；

本校为第一机构

目的：建立HPLC法测定复方苯妥英钠凝胶剂中克林霉素磷酸酯的含量。方法：采用ZORBAX SB-C18色谱柱（250mm×46 mm,5μm）;流动相为0.1 mol·L^-1的磷酸二氢钾（用磷酸调p H 2.5）-乙腈（75∶25）;流速0.8 ml·min^-1;检测波长210 nm;柱温：25℃;进样量10μl。结果：克林霉素磷酸酯在3.00-18.00μg范围内线性关系良好（r=0.999 5）;平均回收率为101.11%（RSD=0.34%,n=6）。结论：本方法简单,灵敏,重复性好,可用于复方苯妥英钠凝胶剂中克林霉素磷酸酯的含量测定。

Objective： To establish an HPLC method for the determination of clindamycin phosphate in compound phenytoin sodium ge1 s. Methods： The HPLC analysis was carried out on a ZORBAX SB-C18column（ 250 × 46 mm,5 μm） with 0. 1 mol · L^-1KH2PO4solution（ adjusting p H to 2. 5 with H3PO4solution）-acetonitrile（ 75 ∶ 25） as the mobile phase at the flow rate of 0. 8 ml·min^-1. The detection wavelength was 210 nm,the column temperature was 25℃ and the injection volume was 10 μl. Results： The linear range of clindamycin phosphate was 3. 00-18. ...