Soybean (Glycine max) is one of the most economically important crops in the world and is used widely for different purposes. Breeding programs have developed new varieties with desired traits, including altered fatty acid profiles and levitated protein content. The breeding process involves the selection of elite genotypes from a large number of experimental lines, which is time-consuming and costly. This study aimed to evaluate the feasibility of a reflective hyperspectral imaging system in classifying the fatty acid content of single soybean seeds for high-throughput phenotyping high oleic ...