期刊论文

Xie, Yunchang

英文

Biotechnology Letters

0141-5492

2019

41

8-9

1033-1041

10.1007/s10529-019-02698-y

NSFCNational Natural Science Foundation of China (NSFC) [41806158, 41406195]; Provincial Natural Science Research Project of Anhui Colleges and Universities [KJ2017A177]; Key Laboratory of Tropical Marine Bio-resources and Ecology, SCSIO, CAS [2018011007]; National Training Program of Innovation and Entrepreneurship for Undergraduates [201710366031]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY17C160001]; research project of Education Department of Zhejiang Province [Y201533256]; research project of Science Technology Department of Zhejiang Province [2016C37036]; Open Project Program of Guangdong Key Laboratory of Marine Materia Medica, SCSIO, CAS [2018011007]

本校为其他机构

Objectives: To improve the bioproductivity of secondary metabolites of marine derived Nocardiopsis flavescens CGMCC 4.5723 by enhancing its riboflavin supplement. Results: The NfRibA, type II guanosine triphosphate (GTP) cyclohydrolase (GCH II) of Nocardiopsis flavescens CGMCC 4.5723, was biochemically identified and showed that NfRibA could efficiently catalyze the first step of riboflavin biosynthesis to hydrolyze GTP into 2, 5-diamino-6-ribosylamino-4(3H)-pyrimidinedione 5′-phosphate (DARPP) with Km value of 160.11 ± 26.81μM in vitro. The...