Wuhan Science and Technology Planning Project [201070934341]
机构署名：
本校为第一且通讯机构
院系归属：
生命科学与技术学院
摘要：
Bacillus cereus strains containing voitoxin cereulide are pathogenic bacteria. The cereulide is produced by B. cereus strains containing plasmid pCER270. To establish a specific loop-mediated isothermal amplification (LAMP) method detecting pathogenic B. cereus, LAMP primer sets were designed based on 16SrDNA and pCER270 plasmid cesA sequence. The LAMP reaction was carried out under optimal conditions with 2 mM of Mg2+ at 65C for 1 h. Specificity of LAMP primers were validated by assaying 19 B. cereus strains with and without pCER270 plasmid ...
