A highly sensitive immunochromatographic assay for lead ions in drinking water based on antibody-oriented probe and silver enhancement

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成果类型：

期刊论文

作者：

Li, Yangyang;Zhu, Zhengwei;Qu, Wenli;Yang, Qing;Liu, Yan;...

通讯作者：

Xu, L

作者机构：

[Qu, Wenli; Li, Yangyang] Wuhan Polytech Univ, Coll Food Sci & Engn, Xuefunan Rd 68, Wuhan 430023, Peoples R China.

[Zhu, Zhengwei] Hubei Prov Inst Food Supervis & Test, Wuhan 430075, Peoples R China.

[Liu, Yan; Wu, Jine; Gong, Zhiyong; Duan, Shuo; Xu, Lin; Wang, Qiao; Yang, Qing] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.

[Liu, Yan; Wu, Jine; Gong, Zhiyong; Duan, Shuo; Xu, Lin; Wang, Qiao; Yang, Qing] Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

[Liu, Yan] Key Res Inst Humanities & Social Sci Hubei Prov, Food Safety Res Ctr, Wuhan 430023, Peoples R China.

通讯机构：

[Xu, L ] M

Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.

Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

语种：

英文

关键词：

Silver enhancement;Antibody-oriented probe;Immunochromatographic assay;Lead ions

期刊：

European Food Research and Technology

ISSN：

1438-2377

年：

2023

卷：

249

期：

页码：

3097-3103

DOI：

10.1007/s00217-023-04351-5

基金类别：

This work has been supported by the National Natural Science Foundation of China (No. 32102072) and the Fundamental Research Project of Wuhan Knowledge Innovation Program (No. 2022020801020399). [2022020801020399]; National Natural Science Foundation of China; Fundamental Research Project of Wuhan Knowledge Innovation Program; [32102072]

机构署名：

本校为第一机构

院系归属：

食品科学与工程学院

摘要：

Lead ions are a toxic metal that can enter the human body through the diet and negatively impact human health. A wide variety of foods, such as vegetables, meat, aquatic product, and drinking water, can be contaminated with lead ions. In this study, a highly sensitive immunochromatographic assay (ICA) based on antibody-oriented probe and silver enhancement was established for the qualitative and quantitative determination of lead ions in drinking water. The recombinant protein A (PrA) was used in probe preparation to improve antibody bioactivit...

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A highly sensitive immunochromatographic assay for lead ions in drinking water based on antibody-oriented probe and silver enhancement

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