期刊论文、会议论文

LPS Induces MuRF1 Transcription through AKT/FOXO1 Mediating Pathway in C2C12 Myotubes

中文

畜牧兽医学报

0366-6964

2016

47

2

374-380

中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会

2016-10-21

中国湖北武汉

10.11843/j.issn.0366-6964.2016.02.022

湖北省自然科学基金(2015CFB514);湖北省高等学校优秀中青年科技创新团队计划项目(T201508)

本校为第一机构

以C2C12肌管细胞为试验材料,探讨脂多糖(LPS)诱导的炎症反应对肌管蛋白降解相关基因及信号通路的影响。选取不同质量浓度(10、100、1 000ng?mL-1)的LPS分别刺激C2C12肌管细胞30min和3h,通过RTqPCR检测炎症因子TNF-α和IL-6的基因转录,确定LPS最佳刺激浓度以建立细胞炎症模型。用最佳浓度LPS分别刺激C2C12肌管细胞0min、30min、1h、3h、6h、12h、24h,RT-qPCR检测MAFbx基因和MuRF基因在不同时间点的转录量变化。此外,1 000ng?mL-1 LPS刺激C2C12肌管细胞12h后,Western blot检测AKT/FOXO1、mTOR和P38信号通路相关蛋白质的表达情况。结果表明:LPS刺激C2C12肌管细胞的最佳浓度为1 000ng?mL-1,在作用30...

This study was designed to investigate the effects of the inflammatory induced by lipopolysaccharide(LPS)on the genes and pathways controlling the protein degradation in C2C12 myotubes.In order to establish an inflammatory cell mode,the C2C12 myotubes were treated with graded concentrations of LPS(10,100 and 1 000ng?mL-1)for 30min and 3h,and then the mRNA transcription of TNF-α and IL-6 were determined by qPCR method.The results showed that 1 000ng?mL-1 LPS was the optimal concentration.After the C2C12 myotubes were treated with 1 000ng?mL-1 L...