期刊论文

Yang, Jiang-Ke

英文

Enzyme and Microbial Technology

0141-0229

2015

71

66-72

10.1016/j.enzmictec.2014.10.007

We thank J. S. Cheng and C. Hu for their help in the conduction of the experiments. This work was financially supported by the Science and Technology Supporting Program of Hubei Province (2014) , the National High Technology Research and Development Program of China (no. 2013AA102805-4 ), the Key Project of Science and Technology Research Program of the Education Department of Hubei Province (no. D20131703 ), and the Key Project of Chinese Ministry of Education (no. 212118 ), where Dr. JK Yang is an incumbent member of the Chutian Scholar Program.

本校为第一且通讯机构

Lipase (EC 3.1.1.3) stands amongst the most important and promising biocatalysts for industrial applications. In this study, in order to realize a high-level expression of the Yarrowia lipolytica lipase gene in Pichia pastoris, we optimized the codon of LIP2 by de novo gene design and synthesis, which significantly improved the lipase expression when compared to the native lip2 gene. We also comparatively analyzed the effects of the promoter types (PAOX1 and PFLD1) and the Pichia expression systems, including the newly developed PichiaPink syst...