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Engineering the 5 ' UTR-Mediated Regulation of Protein Abundance in Yeast Using Nucleotide Sequence Activity Relationships

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成果类型：

期刊论文

作者：

Ding, Wentao;Cheng, Jian;Guo, Dan;Mao, Ling;Li, Jingwei;...

通讯作者：

Jiang, Huifeng

作者机构：

[Lu, Lina; Cheng, Jian; Jiang, Huifeng; Ding, Wentao; Guo, Dan] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China.

[Ding, Wentao] Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing 100029, Peoples R China.

[Mao, Ling; Yang, Jiangke] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

[Zhang, Yunxin; Li, Jingwei] Fudan Univ, Shanghai Key Lab Contemporary Appl Math, Sch Math Sci, Lab Math Nonlinear Sci,Ctr Computat Syst Biol, Shanghai 200433, Peoples R China.

通讯机构：

[Jiang, Huifeng] C

Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China.

语种：

英文

关键词：

5' untranslated region;Saccharomyces cerevisiae;directed evolution;translational regulation

期刊：

ACS Synthetic Biology

ISSN：

2161-5063

年：

2018

卷：

期：

页码：

2709-2714

DOI：

10.1021/acssynbio.8b00127

基金类别：

This work was supported by the National Natural Science Foundation of China (31501041) to W.D. This work was also supported by a 973 Program grant (2015CB755704) and two National Natural Science Foundation of China grants (31470215 and 31670100) to H.J.

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本校为其他机构

院系归属：

生命科学与技术学院

摘要：

The 5′ untranslated region (5′UTR) plays a key role in post-transcriptional regulation, but interaction between nucleotides and directed evolution of 5′UTRs as synthetic regulatory elements remain unclear. By constructing a library of synthesized random 5′UTRs of 24 nucleotides in Saccharomyces cerevisiae, we observed strong epistatic interactions among bases from different positions in the 5′UTR. Taking into account these base interactions, we constructed a mathematical model to predict protein abundance with a precision of R 2 = 0.60. On...

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