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Isolation and pathogenicity of a variant porcine epidemic diarrhea virus field strain with high adaptability to Vero cell

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成果类型:
期刊论文
作者:
Liao, Tianjing;Zhang, Yi;Ma, Huanhuan;Wang, Zhanchang;Zhou, Ao;...
作者机构:
[Liao, Tianjing; Zhang, Yi; Ma, Huanhuan; Zhou, Ao; Zhang, Yu; Zhang, Zhengfan; Guo, Shuangshuang; Hou, Yongqing; Li, Zhonghua; Wu, Tao] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
[Wang, Zhanchang] Forestry and Fruit Tree Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan, China
语种:
英文
关键词:
Porcine epidemic diarrhea virus;G2b subtype;Isolation;Adaptability;pathogenicity
期刊:
Frontiers in Veterinary Science
ISSN:
2297-1769
年:
2025
卷:
12
页码:
1654230
机构署名:
本校为第一机构
院系归属:
动物科学与营养工程学院
摘要:
Since 2010, new outbreaks of porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) variant strains have brought significant economic losses to world pig industry. In this study, we isolated a PEDV strain from a new PED outbreak farm in 2024. The strain was identified through RT-PCR, indirect immunofluorescence assay and purified through plaque assay. This virus showed high adaptability to Vero cell during the process of passage and named as HB-2024.Phylogenetic analysis of the S gene showed that the HB-2024 strain was clustered into G2b subgroup. Amino sequence analy...

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