期刊论文

mRNA expression of lncRNA-44651 and lncRNA-45208 in thymus, lymph nodes and jejunum of piglets induced by LPS

中文

黑龙江畜牧兽医

1004-7034

2020

13

17-21

10.13881/j.cnki.hljxmsy.2019.07.0459

国家自然科学基金项目(31702203) 中央高校基本科研业务费专项(CZZ16003)。

本校为其他机构

为了探究脂多糖（LPS）刺激仔猪发生炎症时炎性因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）、lncRNA-44651和lncRNA-45208在仔猪胸腺、淋巴结和空肠的表达差异,试验选取8头杜×长×大健康断奶仔猪并按照是否注射LPS随机分为对照组和LPS组,各组仔猪在饲喂基础饲粮14 d后,分别按体重100μg/kg腹腔注射生理盐水和LPS,4 h后屠宰仔猪并取胸腺、淋巴结和空肠样品置液氮中保存,采用基因表达测定的方法研究仔猪胸腺、淋巴结和空肠样品相关基因的表达情况。结果表明:LPS刺激4 h后,LPS组仔猪胸腺、淋巴结和空肠中炎性因子TNF-α的mRNA相对表达量均无显著变化（P>0.05）,IL-1β的mRNA相对表达...

To investigate the expression differences of inflammatory factors TNF-α, IL-1β, lncRNA-44651 and lncRNA-45208 in the thymus, lymph nodes and jejunum of piglets when lipopolysaccharide(LPS) stimulated inflammation in piglets, eight healthy weanling Duroc × Landrac × Yorkshire pigletss were randomly divided into control group and LPS group according to whether they were injected with LPS or not. The piglets were fed with basic diet for 14 days, then injected with normal saline or LPS according to their body weight of 100 μg/kg intraperitonea...