Using the defatted and desquamated rapeseed meal as material, a kind of nature mixed amino acids was prepared by hydrolyzing the material with sulfuric acid under higher temperature for short time. The result of orthogonal experiment indicated that the optimum reaction conditions were as followed: reaction temperature 130℃, reaction time 2h, concentration of sulfuricacid 6mol/L, with the rate of liquid and solid 3 : 1. After neutralizing by CaCO3, concentrating and freezing dryness ,the yield of nature mixtured amino acids was 38.70%. The am...