期刊论文

Establishment of Experimental Model for Colostrum-deprived Piglets Infected by Haemophilus Parasuis

中文

家畜生态学报

1673-1182

2015

36

06

53-62

10.3969/j.issn.1673-1182.2015.06.012

国家自然科学基金项目(31301939)； 武汉市科技局应用基础研究计划项目(2013020501010178)； 湖北省自然科学基金项目(2013CFB324)；

本校为第一机构

为了利用禁食初乳(colostrum-deprived,CD)仔猪构建可靠的猪格拉泽氏病实验动物模型,本研究分别选取12头新生通城仔猪和15头新生杜长大仔猪,母猪自然分娩后对新生仔猪立即隔离并做必要消毒处理后实施人工喂养;45~46日龄,CD仔猪经气管内接种致死剂量(109 CFU)的副猪嗜血杆菌(0165菌株,血清型5)。结果显示:⑴利用本研究优化、建立的饲喂方案使得CD仔猪攻毒前存活率达到80%~83.3%;⑵所有通城CD仔猪经攻毒后临床上表现出典型的格拉泽氏病病征,其中66.7%的CD仔猪可于攻毒后存活3d;而杜长大CD仔猪可能由于存在猪胸膜肺炎放线杆菌感染导致攻毒后无明显病征;⑶通城CD仔猪剖检后明显可见纤维素性心包炎、...

To develop the reliable experimental model of Glsser＇s disease in colostrum-deprived（CD）piglets,a total of 12 newborn Tongcheng piglets and 15 newborn Duroc×Landrace×Large white piglets were selected after natural delivery.Piglets were collected immediately after birth and were artificially reared in a clean research facility.At the age of 45-46 d,CD piglets from both breeds were inoculated intratracheally（IT）with lethal does（109CFU）of Haemophilus Parasuis（HPS）serovar 5（strain 0165）.The results showed that：⑴ 80%-83.3% experimen...